E11 Bio is a nonprofit Focused Research Organization founded in 2021 and headquartered in Alameda, California. The organization develops PRISM, a scalable technology platform for brain circuit mapping that combines protein barcoding, advanced microscopy, and machine learning to reconstruct neural connectivity at single-cell resolution in mammalian brains. E11 Bio serves the neuroscience research community, collaborating with academic institutions and for-profit companies to advance connectomics research and support applications in drug discovery, brain-inspired computing, and therapeutic development. The organization is led by Co-founder and CEO Andrew Payne and Chief Operating Officer Jun Axup Penman, alongside Lead Scientists Johan Winnubst (Neuroanatomy) and Kathleen Leeper (Barcoding). E11 Bio is philanthropically funded and operates with an estimated 11–20 employees.